Thursday, December 21, 2006

GSD-10 mixing instructions

To make GSD-10 you'll need a 1 liter mixing container, the constituent chemicals, and standard lab safety equipment, including; dust mask or respirator, lab glasses and gloves. Work in a well ventilated area.

GSD-10 is made up acording to standard lab practices. Start by adding 750ml of distilled water to your mixing container. Add with stirring, the constituent chemicals in the order they appear in the formula, and be sure each chemical is completely dissolved before adding the next. When all of the chemicals have been dissolved, top up to 1 liter with distilled water. Your concentrated stock solution is now ready to use. Dilute 1:10* to make a working solution.

* Dilution ratios are written in the form X:Y. X = stock solution, and Y = total solution volume. To make a 1:10 dilution, simply divide the total volume by 10 to find the correct volume of stock solution. V/10=VSS (volume divided by ten equals volume of stock solution)


To make 1 liter- 1000/10=100ml stock solution

To make 300ml- 300/10=30ml stock solution



Anonymous said...

I have a bit more fresh glycin than I need to make up 1 liter of GSD10.

Would I be better off mixing up the remainder and storing it in airtight brown glass bottles (and hoping that the Sodium Sulfite preserves it), OR
wrapping the packaged Glycin in aluminum foil, vacuum sealing it and storing it in the fridge?

cheers - Tom

John Finch said...

It's perhaps a little late now to reply to this but vacuum sealing glycin and freezing it will keep it for a year or so.

jdef said...

Thanks for the tip, John.

Kevin McGovern said...

Is it correct to say that the term 1:10 = "One part in ten", meaning 1 part solute and nine parts solvent to make up a total volume of ten parts? Thus, to make 1 liter of working solution, one would add 100mL of GSD-10 stock into a volume of 900mL distilled water for a total volume of 1000mL, or 1 liter.

We often see dilutions notated as 1+10 and 1:10 used interchangeably, which is wrong, given the former should result in 1000mL, the latter, 1100mL.

Seems I read many moons back 1:10 in organic chemistry meant "1 IN 10", and inorganic chemistry indicated "1 Plus 10". So much for armchair chemistry...